Micropipette

The pipette's aperture can be as low as 0.1μm, but the tip is very fragile, so it is easy to be damaged in the process of sample preparation. It is necessary to be careful of the tip damage when preparing, and ensure that the pipette's surrounding seal is intact after implantation.  After the surrounding seal is completed with epoxy resin, the seal's integrity is verified by microscopic observation.  Tolerance of taper tip diameter is usually ±20%.  For example, the channel length of the above 2μm glass microfiber is difficult to define. It is generally assumed that it is shorter than 0.5mm.  In addition, residual air can interfere with the test results.

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The tip of a Micro pipette is used to create defects of a certain diameter

Flow calibration is used to authenticate leaks

Geometry of tip defects